Mouse CCL21a Matched Antibody Pair Kit (ab216060)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Mouse CCL21a Matched Antibody Pair Kit
See all Ccl21a kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.9 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CCL21a Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CCL21a.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208985. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CCL21a Capture Antibody 2 x 50µg 1 x 50µg Mouse CCL21a Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CCL21a Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
Tissue specificity
Expressed strongly in lung, spleen, thymus, peripheral and mesentric lymph nodes. Also expressed in the testis, kidney, liver, and heart. -
Involvement in disease
Note=Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 6Ckine
- Beta-chemokine exodus-2
- C-C motif chemokine 21a
see all -
Database links
- Entrez Gene: 100504362 Mouse
- Entrez Gene: 18829 Mouse
- SwissProt: P84444 Mouse
- Unigene: 220853 Mouse
Images
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Mouse CCL21a standard curve.
Standard calibration curve. Background subtracted values are graphed.
