Mouse CCL21a ELISA Kit (ab208985)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CCL21a ELISA Kit
See all Ccl21a kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.1% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
3.91 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115 111% - 116% Cell culture media 109 106% - 113% Hep Plasma 101 95% - 107% EDTA Plasma 104 101% - 106% Cit plasma 111 107% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse CCL21a ELISA Kit (ab208985) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL21a protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL21a with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse CCL21a is a member of the CC subfamily of chemokines. Chemokines are a class of cytokines that induce chemotaxis of target cells. CCL21a is highly expressed in the lymph nodes and chemoattracts naïve T cells and mature dendritic cells into T zones of secondary lymphoid organs. Furthermore, it has been suggested that CCL21a induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of soluble or immobilized CCL21a inhibits T cell proliferation and IL-2 secretion. Human CCL21a exhibits 71% amino acid identity to mouse CCL21a.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CCL21a Capture Antibody 1 x 600µl 10X Mouse CCL21a Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse CCL21a Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
Tissue specificity
Expressed strongly in lung, spleen, thymus, peripheral and mesentric lymph nodes. Also expressed in the testis, kidney, liver, and heart. -
Involvement in disease
Note=Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 6Ckine
- Beta-chemokine exodus-2
- C-C motif chemokine 21a
see all -
Database links
- Entrez Gene: 100504362 Mouse
- Entrez Gene: 18829 Mouse
- SwissProt: P84444 Mouse
- Unigene: 220853 Mouse
Images
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Other - Mouse CCL21a ELISA Kit (ab208985)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CCL21a standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CCL21a standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CCL21a in mouse serum and plasma samples.The concentrations of CCL21a were measured in duplicates, interpolated from the CCL21a standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, and plasma (heparin) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL21a concentration was determined to be 6.51 ng/mL in serum, 7.49 ng/mL in plasma (citrate), 5.97 ng/mL in plasma (EDTA) and 6.07 ng/mL in plasma (heparin).
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Interpolated concentrations of native CCL21a in mouse lung extract and mouse lung supernatant samples.Interpolated concentrations of native CCL21a in mouse lung extract based on a 100 μg/mL extract load. The concentrations of CCL21a were measured in duplicates, interpolated from the CCL21a standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse lung extract 100 μg/mL and mouse lung supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL21a concentration was determined to be 0.61 ng/mL in mouse lung extract and 3.15 ng/mL in mouse lung supernatant. Mouse lung supernatant was cultured for 6 days in RPMI base media plus 10% fetal bovine serum (FBS).
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Sandwich ELISA - Mouse CCL21a ELISA Kit (ab208985)To learn more about the advantages of recombinant antibodies see here.
