Mouse Biglycan Antibody Pair - BSA and Azide free (ab253458)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Biglycan Antibody Pair - BSA and Azide free
See all Biglycan kits -
Assay type
ELISA set -
Range
546.875 pg/ml - 35000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Mouse Biglycan. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab275906), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Biglycan Capture Antibody (unconjugated) 1 x 100µg Mouse Biglycan Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
May be involved in collagen fiber assembly. -
Tissue specificity
Found in several connective tissues, especially in articular cartilages. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- BGN
- Biglycan
- Biglycan proteoglycan
see all -
Database links
- Entrez Gene: 12111 Mouse
- SwissProt: P28653 Mouse
- Unigene: 2608 Mouse
Images
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Sandwich ELISA - Mouse Biglycan Antibody Pair - BSA and Azide free (ab253458)To learn more about the advantages of recombinant antibodies see here.
