Mouse BAFF ELISA Kit (ab216948)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.4 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse BAFF ELISA Kit
See all BAFF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 8 3.3% Inter-assay Sample n Mean SD CV% Mouse serum 3 1.8% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.4 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 101% - 103% Hep Plasma 96 91% - 101% EDTA Plasma 98 95% - 102% Cit plasma 98 91% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse BAFF ELISA Kit (ab216948) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAFF protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse BAFF with 7.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
B-cell activating factor (BAFF) protein is a 309 amino acid cytokine encoded by the TNFSF13B gene. BAFF has been previously referred to as B Lymphocyte Stimulator (BLys), TNF- and APOL- related leukocyte expressed ligand (TALL-1), and Dendritic cell-derived TNF-like molecule (CD257 antigen). BAFF protein is a member of the tumor necrosis factor (TNF) superfamily of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. It is expressed as a type II transmembrane protein on various cell types including monocytes, dendritic cells, and bone marrow stromal cells. The extracellular portion can be cleaved from the membrane, generating a soluble protein fragment. Inadequate levels of BAFF fail to activate B-cells to produce enough immunoglobulin and lead to immunodeficiency. Excessive levels of BAFF cause abnormally high antibody production, resulting in systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse BAFF Capture Antibody 1 x 600µl 10X Mouse BAFF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse BAFF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
Database links
- Entrez Gene: 24099 Mouse
- SwissProt: Q9WU72 Mouse
- Unigene: 28835 Mouse
Images
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Other - Mouse BAFF ELISA Kit (ab216948)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse BAFF standard curve comparison dataStandard curve comparison between mouse BAFF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of mouse BAFF standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native BAFF in mouse serum and plasma samples.The concentrations of BAFF were measured in duplicate, interpolated from the BAFF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BAFF concentration was determined to be 22 ng/mL in serum, 16 ng/mL in plasma (citrate), 20 ng/mL in plasma (EDTA), and 24 ng/mL in plasma (heparin).
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Sandwich ELISA - Mouse BAFF ELISA Kit (ab216948)To learn more about the advantages of recombinant antibodies see here.
