Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.1 -
Assay duration
Multiple steps standard assay -
Product overview
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326) provides a quick, sensitive and easy way for measuring MAGL activity in various samples. In this assay, a fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolite and the increased fluorescence is measured at Ex/Em 360/460 nm. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence.
The assay is simple to perform, high throughput adaptable and can detect as low as 0.1 mU of MAGL activity.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests MAGL Assay Buffer 1 x 25ml MAGL Positive Control (lyophilized) 1 vial MAGL Selective Inhibitor (200X) 1 x 100µl MAGL Substrate 1 x 50µl Umbelliferone Standard (10 mM) 1 x 40µl -
Research areas
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Function
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
Tissue specificity
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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Alternative names
- EC 3.1.1.23
- HU K5
- HU-K5
see all
Images
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Fluorescent standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Fluorescent standard curve.
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Kinetic reactions of MAGLReaction kinetics of Monoacylglycerol Lipase (MAGL) positive control and MAGL activity in rat midbrain lysate (6 µg protein) using appropriate background controls.
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Monoacylglycerol Lipase specific activityMonoacylglycerol Lipase specific activity was calculated in rat midbrain and rat intestinal lysates.
