MMP7 Inhibitor Screening Assay Kit (Fluorometric) (ab139445)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
MMP7 Inhibitor Screening Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Abcam MMP7 Inhibitor Screening Assay Kit (Fluorometric) (ab139445) is a complete assay system designed to screen MMP7 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.
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Notes
This kit is useful to screen inhibitors of MMP7, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 96-well White Microplate 1/2 Volume 1 unit Fluorometric Assay Buffer 1 x 20ml MMP Calibration Standard 1 x 50µl MMP Fluorogenic Substrate 1 x 200µl MMP Inhibitor 1 x 50µl MMP7 Enzyme (Human, Recombinant) 1 x 33.3µl -
Research areas
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Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Matrilysin
- Matrin
- Matrix Metalloproteinase 7
see all
Images
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Example of Inhibitor Data