Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter (ab112144)
Overview
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Product name
Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter
See all Mitochondria kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Mitochondrial Staining Kit - Orange Fluorescence | Cytopainter (ab112144) is designed to label mitochondria in live cells with orange fluorescence at Ex/Em = 545/575nm (TRITC filter-compatible). This kits uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. ab112144 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry.
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml MitoOrange Indicator 1 x 100µl -
Research areas
Images
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Fluorescence Microscopy - CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144)
CytoPainter Mitochondrial Staining Kit - Orange Fluorescence (ab112144). Image of U2OS cells stained with ab112144 in a black 96-well plate