MitoBiogenesis™ Western Blot Cocktail (ab123545)
Overview
-
Product name
MitoBiogenesis™ Western Blot Cocktail -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.
Cocktail Antibodies:
Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse Beta actin [mAbcam 8224] monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/ml
-
Notes
Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
-
Tested applications
Suitable for: WBmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 µg Antibody Cocktail 1 x 200µl -
Research areas
-
Database links
- SwissProt: P23219 Human
- SwissProt: P60709 Human
- SwissProt: P31040 Human
Images
-
Western blot - Biogenesis Western Blot Cocktail (ab123545)All lanes : MitoBiogenesis™ Western Blot Cocktail (ab123545) at 1/250 dilution
Lane 1 : Human HepG2 cell lysate
Lane 2 : Human Hela cell lysate
Lane 3 : Human HDFn cell lysate
Lane 4 : Human SH-SY5Y cell lysate
Lane 5 : Rat H9C2 cell lysate
Lane 6 : Rat H4IIE cell lysate
Lane 7 : Mouse 3T3 cell lysate
Lane 8 : Mouse MEF cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : GAM-HRP at 1/3000 dilution
-
Western blot - Biogenesis Western Blot Cocktail (ab123545)All lanes : MitoBiogenesis™ Western Blot Cocktail (ab123545) at 1/250 dilution
Lane 1 : Human HepG2 cell lysate (vehicle treated)
Lane 2 : Human HepG2 cell lysate (Chloramphenicol treated)
Lane 3 : Human HepG2 cell lysate (DDC treated)
Lane 4 : Human 143B WT cell lysate
Lane 5 : Human 143B p0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : GAM-HRP at 1/3000 dilution