Methylglyoxal ELISA Kit (ab238543)
Key features and details
- Assay type: Competitive
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
Overview
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Product name
Methylglyoxal ELISA Kit
See all Methylglyoxal kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Competitive -
Range
0.2 µg/ml - 25 µg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Vertebrata -
Product overview
Methylglyoxal ELISA Kit (ab238543) is developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.
The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
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Tested applications
Suitable for: Competitive ELISAmore details -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 100X Conjugate Diluent 1 x 300µl 10X Wash Buffer 1 x 100ml Protein Binding Strip Well Plate 1 unit Anti-MG Antibody (1000X) 1 x 10µl Assay Diluent 1 x 50ml MG Conjugate 1 x 20µl MG-BSA Standard 1 x 75µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl Stop Solution 1 x 12ml Substrate Solution 1 x 12ml -
Research areas
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Relevance
Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms. -
Alternative names
- 2 oxo propanal
- MG
- pyruvaldehyde
Images
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Example MG-BSA Competitive ELISA Standard Curve.
Typical Methylglyoxal (MG) Competitive ELISA results.
This data is for reference only and should not be used to interpret actual results.
