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Signal Transduction Metabolism Energy Metabolism

Methylglyoxal ELISA Kit (ab238543)

Price and availability

499 209 ₸

Availability

Order now and get it on Thursday February 25, 2021

Methylglyoxal ELISA Kit (ab238543)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Assay type: Competitive
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate

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Overview

  • Product name

    Methylglyoxal ELISA Kit
    See all Methylglyoxal kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
  • Assay type

    Competitive
  • Range

    0.2 µg/ml - 25 µg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Vertebrata
  • Product overview

    Methylglyoxal ELISA Kit (ab238543) is developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.


    The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    100X Conjugate Diluent 1 x 300µl
    10X Wash Buffer 1 x 100ml
    Protein Binding Strip Well Plate 1 unit
    Anti-MG Antibody (1000X) 1 x 10µl
    Assay Diluent 1 x 50ml
    MG Conjugate 1 x 20µl
    MG-BSA Standard 1 x 75µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl
    Stop Solution 1 x 12ml
    Substrate Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Relevance

    Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms.
  • Alternative names

    • 2 oxo propanal
    • MG
    • pyruvaldehyde

Images

  • Example MG-BSA Competitive ELISA Standard Curve.
    Example MG-BSA Competitive ELISA Standard Curve.

    Typical Methylglyoxal (MG) Competitive ELISA results.

    This data is for reference only and should not be used to interpret actual results.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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