Metformin hydrochloride, AMPK activator (ab120847)
Key features and details
- AMPK activator
- CAS Number: 1115-70-4
- Purity: > 99%
- Soluble in water to 100 mM and in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Metformin hydrochloride, AMPK activator -
Description
AMPK activator -
Biological description
AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.
Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 99% -
CAS Number
1115-70-4 -
Chemical structure
Properties
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Chemical name
N,N-Dimethylimidodicarbonimidic diamide hydrochloride -
Molecular weight
165.63 -
Molecular formula
C4H11N5.HCl -
PubChem identifier
14219 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM and in DMSO to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN(C)C(=N)N=C(N)N.Cl -
Source
Synthetic
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Research areas
Images
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Functional Studies - Metformin hydrochloride, AMPK activator (ab120847)
ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.