Clotrimazole, Non-selective P450 cytochrome inhibitor (ab120744)
Key features and details
- Non-selective P450 cytochrome inhibitor
- CAS Number: 23593-75-1
- Purity: > 99%
- Soluble in DMSO to 75 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Clotrimazole, Non-selective P450 cytochrome inhibitor -
Description
Non-selective P450 cytochrome inhibitor -
Alternative names
- CLT
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Biological description
Non-selective P450 cytochrome inhibitor (Ki = 0.02-67 μM for P450 cyctochrome subtypes). Potent inhibitor of 34A (Ki = 20 nM). Antifungal agent.
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Purity
> 99% -
CAS Number
23593-75-1 -
Chemical structure
Properties
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Chemical name
1-(2-Chloro-α,α-diphenylbenzyl)imidazole -
Molecular weight
344.84 -
Molecular formula
C22H17ClN2 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 75 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Clotrimazole, Non-selective P450 cytochrome inhibitor (ab120744)
HepG2 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of clotrimazole (ab120744)in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.