MCF7 membrane extract lysate (ab29539)
Overview
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Product name
MCF7 membrane extract lysate
See all MCF7 lysates -
General notes
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
Storage buffer
Constituents: 0.1% Bromophenol blue, 0.1% Magnesium chloride, 1.17% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.1% HEPES, 0.1% EDTA, 10% Glycerol, 0.1% Sodium chloride -
Concentration information loading... -
Lysate notes
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
Research areas
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Background
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
