MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)
Overview
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Product name
MCF7 nuclear extract lysate (H2O2 post treated)
See all MCF7 lysates -
Description
MCF7 nuclear extract lysate (H2O2 post treated) -
General notes
Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
Images
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Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)
Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5
µ g/well must be above 4.
