Malate Assay Kit (ab83391)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Food samples, Tissue Extracts
- Sensitivity: 20 µM
Overview
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Product name
Malate Assay Kit -
Detection method
Colorimetric -
Sample type
Tissue Extracts, Food samples -
Assay type
Quantitative -
Sensitivity
20 µM -
Range
20 µM - 200 µM -
Assay time
1h 00m -
Product overview
Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect 1~10 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.
Visit our FAQs page for tips and troubleshooting. -
Notes
L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Malate assay buffer WM 1 x 20ml Malate Enzyme Mix (Lyophilised) Green 1 vial Malate Standard (10 µmol) (Lyophilized) Yellow 1 vial WST Substrate (Lyophilised) Red 1 vial -
Research areas
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Relevance
L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others. -
Alternative names
- Malic acid
Images
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Example of Standard Curve