Anti-NPSR1 antibody (ab92425)
Key features and details
- Rabbit polyclonal to NPSR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NPSR1 antibody
See all NPSR1 primary antibodies -
Description
Rabbit polyclonal to NPSR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human NPSR1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab101418) -
Positive control
- This antibody gave a positive signal in Human Heart Tissue Lysate. IHC-P (FFPE): Human Colon (Normal)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab92425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ICC/IF Use a concentration of 5 µg/ml. Target
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Relevance
Neuropeptide S receptor 1 (GPR154) is a receptor for Neuropeptide S. It is expressed on the epithelia of several organs including the intestine, and appears to be upregulated in inflammation. It is upregulated in macrophages after antigen challenge and is involved in the pathogenesis of asthma and other IgE-mediated diseases. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 387129 Human
- Entrez Gene: 319239 Mouse
- Entrez Gene: 300458 Rat
- Entrez Gene: 574355 Rhesus monkey
- Omim: 608595 Human
- SwissProt: Q6W5P4 Human
- SwissProt: Q8BZP8 Mouse
- SwissProt: P0C0L6 Rat
see all -
Alternative names
- ASRT2 antibody
- G protein coupled receptor 154 antibody
- G protein coupled receptor for asthma susceptibility antibody
see all
Images
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Western blot - Anti-NPSR1 antibody (ab92425)Anti-NPSR1 antibody (ab92425) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-NPSR1 antibody (ab92425)ICC/IF image of ab92425 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92425, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPSR1 antibody (ab92425)IHC image of NPSR1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92425, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
References (3)
ab92425 has been referenced in 3 publications.
- Zhao P et al. Neuropeptide S Ameliorates Cognitive Impairment of APP/PS1 Transgenic Mice by Promoting Synaptic Plasticity and Reducing Aß Deposition. Front Behav Neurosci 13:138 (2019). PubMed: 31293402
- Slattery DA et al. Selective breeding for high anxiety introduces a synonymous SNP that increases neuropeptide S receptor activity. J Neurosci 35:4599-613 (2015). PubMed: 25788677
- Zhang S et al. Persistent nociception induces anxiety-like behavior in rodents: role of endogenous neuropeptide S. Pain 155:1504-15 (2014). PubMed: 24793908
Images
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Western blot - Anti-NPSR1 antibody (ab92425)Anti-NPSR1 antibody (ab92425) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-NPSR1 antibody (ab92425)ICC/IF image of ab92425 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92425, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPSR1 antibody (ab92425)
IHC image of NPSR1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92425, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
