Lysosomal Staining Kit - NIR Fluorescence - Cytopainter (ab176746)
Overview
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Product name
Lysosomal Staining Kit - NIR Fluorescence - Cytopainter
See all Lysosomes kits -
Sample type
Suspension cells -
Assay type
Cell-based (qualitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Lysosomal Staining Kit - NIR Fluorescence | Cytopainter (ab176746) is a part of a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
CytoPainter Lysosomal Staining Kit - NIR Fluorescence (ab176746) is designed to label lysosomes of live cells in near infrared red fluorescence at Ex/Em = 630/650 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. LysoNIR Indicator is used in the kit has extremely high photostability as well as excellent cellular retention makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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Notes
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml LysoNIR Indicator 1 x 100µl -
Research areas
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Alternative names
- lysosome marker
- lysotracker
Images
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Image of HeLa cells stained with CytoPainter Lysosomal Staining Kit - NIR Fluorescence (ab176746)
Image of HeLa cells stained with Abcam's CytoPainter Lysosomal Staining Kit – NIR Fluorescence (ab176746) in a Costar black 96-well.
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Excitation and Emission spectra of CytoPainter Lysosomal Staining - NIR Fluorescence (ab176746)