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Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)

Price and availability

160 819 ₸

Availability

Order now and get it on Thursday February 25, 2021

Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
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Overview

  • Product name

    Lysosomal Staining Kit - Blue Fluorescence - Cytopainter
    See all Lysosomes kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    Lysosomal Staining Kit - Blue Fluorescence | Cytopainter (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. Ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.


    Ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. Ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).


    Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide

  • Notes

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    Live Cell Staining Buffer 1 x 50ml
    LysoBlue Indicator 1 x 100µl
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Lysosome Staining Kits
  • Alternative names

    • lysosome marker
    • lysotracker

Images

  • Immunocytochemistry/ Immunofluorescence - Lysosomal Staining Kit - Blue Fluorescence (ab112135)
    Immunocytochemistry/ Immunofluorescence - Lysosomal Staining Kit - Blue Fluorescence (ab112135)
    ab112135 staining U2OS cells by Immunofluorescence (in a black 96 well plate).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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