Lyophilized MCF7 Exosome Standards (ab239691)
Overview
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Product name
Lyophilized MCF7 Exosome Standards
See all Exosome kits -
Species reactivity
Reacts with: Human -
Product overview
Lyophilized MCF7 Exosome Standards (ab239691) are lyophilized exosomes (1x1012) derived from human cancer cell line. MCF7 is a human breast cancer cell line.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 µg Lyophilized MCF7 Exosome Standards 1 x 100µg -
Relevance
Exosomes are best defined as extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. This liberates intraluminal vesicles (ILVs) into the extracellular milieu and the vesicles thereby released are what we know as exosomes. They contain abundant proteins, DNA, mRNA, and non-coding RNAs. They are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material. Increasing studies have shown that exosomes play an important role in tumour initiation, growth, progression, metastasis, drug resistance and immune escape.
Images
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Dynamic range of fresh (A) and lyophilized (B) exosomes analyzed by flow cytometry.Relationship between background noise and specific signal at different exosome concentrations. Exosomes were captured by CD63+ (Clone TEA3/18) capture beads and subsequently detected by Anti-CD9 PE (Clone VJ1/20).
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Western blot - Lyophilized MCF7 Exosome Standards (ab239691)Fresh and lyophilized Exosome batches were analyzed and compared by WB in native conditions for exosomal markers, by anti-CD9 (Clone VJ1/20) and anti-CD63 (Clon TEA3/18) antibodies at a 1:1000 dilution (0.1 mg/ml).
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Exosome analysis and comparative of fresh (A) and lyophilized (B) exosomes for particle size and concentration by NTAAnalysis was carried out with 1 µl of purified exosomes diluted in 999 µl of HEPES buffer (diution 1:1000). The purified exosomes showed a size distribution profiles, with peak diameters from 50 - 150 nm and concentrations about 1x1010 exosomes/ml.
