Luciferase Reporter Assay Substrate Kit - Renilla (ab228546)
Key features and details
- Detection method: Luminescent
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts
Overview
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Product name
Luciferase Reporter Assay Substrate Kit - Renilla
See all Luciferase Reporter kits -
Detection method
Luminescent -
Sample type
Cell culture extracts, Adherent cells -
Product overview
Luciferase Reporter Assay Substrate Kit - Renilla (ab228546) provides a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. The formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The “glow-type” signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes.
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Notes
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 units 100X Luciferase Substrate 1 x 100µl Assay Buffer 1 x 10ml
Images
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Luciferase titration curve
Renilla Luciferase was measured with Luciferase Reporter Assay Substrate Kit – Renilla (ab228546) in a white 96-well plate.
