LNCaP nuclear extract lysate (testosterone treated) (ab14858)
Overview
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Product name
LNCaP nuclear extract lysate (testosterone treated)
See all LNCaP lysates -
Description
LNCaP nuclear extract lysate -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Cells were cultured in medium supplemented with 10 nM Testosterone for 6 hours at 37°C immediately prior to harvesting.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
The LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer.