Lipid Peroxidation Assay Kit (Cell-based) (ab243377)
Key features and details
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
Overview
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Product name
Lipid Peroxidation Assay Kit (Cell-based)
See all Lipid Peroxidation kits -
Detection method
Fluorescent -
Product overview
Lipid Peroxidation Assay Kit (Cell-based) (ab243377) uses a sensitive ratiometric Lipid Peroxidation Sensor that changes its fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation. Our kit includes H2O2 as a positive control treatment to induce lipid peroxidation.
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Tested applications
Suitable for: Flow Cyt, FMmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests 3% H2O2 1 x 100µl HHBS 1 x 50ml Ratiometric lipid peroxidation sensor R590/G525 (500X) 1 x 50µl
Images
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HeLa cells were stained with 1X Lipid Peroxidation Sensor for 30 minutes in complete growth medium at 37°C.
For H2O2 treatment, approximately 250 µM H2O2 was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid peroxidation Sensor, and stained with Hoechst 33342 during the last 10 minutes of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H2O2 treatment, a clear shift of fluorescence signal of red to green was observed.
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Jurkat cells were stained with 1X Lipid Peroxidation Reagent for 30 minuts in complete growth medium at 37°C.For H2O2 treatment, approximately 250 µM H2O2 was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid Peroxidation Sensor, and analyzed with a flow cytometer through FITC (488/530 nm) and PE (488/572 nm) channels. The data are represented as the ratios of red (PE)/green (FITC) fluorescence intensities. The ratio of red/green decreases in H2O2 treated cells indicating the presence of H2O2-induced lipid peroxidation.
