Lipase Assay Kit III (Fluorometric) (ab118969)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.0001 mU/well
Overview
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Product name
Lipase Assay Kit III (Fluorometric)
See all Lipase kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
> 0.0001 mU/well -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 µU/well.
The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Lipase Assay Buffer WM 1 x 25ml Lipase Positive Control (lyophilized) Purple 1 vial Lipase Substrate Red 1 x 200µl Methylresorufin Standard (0.1 mM) Yellow 1 x 40µl -
Research areas
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Relevance
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
Images
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Functional Studies - Lipase Detection Kit III (ab118969)Positive Control and Background assayed using ab118969.
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Functional Studies - Lipase Detection Kit III (ab118969)Example of standard curve obtained using ab118969.
