Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)
Key features and details
- Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
- CAS Number: 87081-35-4
- Purity: > 97%
- Supplied in ethanol (25 μg/ml)
- Form / State: Liquid
- Source: Steptomyces sp.
Overview
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Product name
Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
Description
Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
Biological description
Potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Displays antibiotic, antifungal, and antitumor properties and blocks the cell cycle at G1 and G2 phases. Potent inhibitor of nucleo-cytoplasmic translocation of Rev. Also shown to suppress HIV-1 replication in human monocytes (IC50 = 600 pM).
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Purity
> 97% -
General notes
Supplied in ethanol (25 µg/ml).
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CAS Number
87081-35-4 -
Chemical structure
Properties
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Chemical name
(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid -
Molecular weight
540.74 -
Molecular formula
C33H48O6 -
PubChem identifier
6917907 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in ethanol (25 μg/ml) -
Handling
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O)C=C(/C)C[C@H](C)[C@@H](O)[C@H](C)C(=O)[C@H](C)C=C(/C)C=CC[C@@H](C)/C=C(C=C[C@@H]1OC(=O)C=C[C@@H]1C)CC -
Source
Steptomyces sp.
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Research areas
Images
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Functional Studies - Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
