Legionella Pneumophila IgM ELISA kit (ab247198)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Legionella Pneumophila IgM ELISA kit
See all Legionella pneumophila kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% sample 24 0.461nM 4.23% sample 24 1.003nM 2.12% sample 24 0.862nM 2.65% Inter-assay Sample n Mean SD CV% sample 12 21.35nM 5.1% sample 12 15.46nM 7.62% sample 12 4.22nM 11.86% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Legionella pneumophila IgM ELISA kit (ab247198) is designed for the qualitative determination of IgM class antibodies against Legionella pneumophila in human serum or plasma (citrate, heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
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Notes
Legionellae are aerobic gram-negative facultative intracellular parasites of certain protozoa. They are found in freshwater environments worldwide and can cause respiratory disease (legionellosis) in humans. L. pneumophila multiplies itself at temperatures between 25 and 42 °C, with an optimal growth temperature of 35 °C. Legionella thrives in warm, stagnant water in the environment and in artificial systems such as cooling towers, evaporative condensers, hot and cold water systems and spa pools that mimic the natural environment in which the organism thrives. These systems also provide the means by which aerosols/droplets are generated and the organism dispersed into the atmosphere.
Legionellosis can be acquired by the inhalation of aerosols containing Legionella bacteria or by micro-aspiration of ingested water contaminated with Legionella. Person-to-person transmission is not thought to be a risk.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgM HRP conjugate 1 x 20ml Cover Foil 1 unit IgM Cut-off Control 1 x 3ml IgM Negative Control 1 x 2ml IgM Positive Control 1 x 2ml IgM Sample Diluent 1 x 100ml Legionella pneumophila Coated Microplate (IgM) 1 unit Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
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Relevance
Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression. -
Database links
- Omim: 608556 Human
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
