L-Arginine Assay Kit (ab241028)
Key features and details
- Detection method: Colorimetric
Overview
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Product name
L-Arginine Assay Kit -
Detection method
Colorimetric -
Product overview
The L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm).
The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Arginine Assay Buffer 1 x 25ml Arginine Enzyme Mix 1 vial Arginine Probe Mix A 1 x 12ml Arginine Probe Mix B 1 x 12ml Arginine Standard 1 vial Sample Cleanup Mix 1 vial
Images
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L-arginine standard curve.L-arginine standard curve.
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Specificity of the detection of L-arginine over other amino acids.Specificity of the detection of L-arginine over other amino acids: D-arginine and other L-amino acids were tested at a 2-fold molar excess (each AA: 40 nmol) vs L-arginine (20 nmol). -
Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL).Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.