JNK Activity Assay Kit (ab273417)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Platform: Western blot
- Sample type: Adherent cells, Cell Lysate, Suspension cells
Overview
-
Product name
JNK Activity Assay Kit
See all c-Jun kits -
Sample type
Adherent cells, Suspension cells, Cell Lysate -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
JNK Activity Assay Kit (ab273417) utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: WBmore details -
Platform
Western blot
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 40 tests c-Jun Protein/ATP Mixture 1 x 80µl JNK Specific Antibody 1 x 80µl Kinase Assay Buffer 1 x 25ml Kinase Extraction Buffer 1 x 80ml Phospho-cJun Specific Antibody 1 x 50µl Protein A Sepharose 1 x 2ml -
Research areas
-
Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localization
Nucleus. - Information by UniProt
-
Alternative names
- Activator protein 1
- AP 1
- AP-1
see all -
Database links
- Entrez Gene: 3725 Human
- Entrez Gene: 16476 Mouse
- Entrez Gene: 24516 Rat
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P05627 Mouse
- SwissProt: P17325 Rat
- Unigene: 696684 Human
see all
Images
-
Western blot analysis Phospho-c-Jun
Western blot analysis of Phospho-c-Jun in various amounts of Jurkat cell lysate.
Lane 1: 50 µg
Lane 2: 100 µg
Lane 3: 200 µg
Lane 4: 400 µg.