Isocitrate Assay Kit (ab83424)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Food samples, Tissue Lysate
- Sensitivity: 0.2 µg
Overview
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Product name
Isocitrate Assay Kit -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate, Food samples -
Assay type
Quantitative -
Sensitivity
> 0.2 µg -
Range
0.2 µg - 5 µg -
Assay time
0h 40m -
Product overview
Isocitrate Assay Kit (ab83424) provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In this assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. This assay can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
Visit our FAQs page for tips and troubleshooting. -
Notes
Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Isocitrate Assay Buffer WM 1 x 25ml Isocitrate Enzyme Mix Green 1 x 200µl Isocitrate Standard (100 mM) (Lyophilised) Yellow 1 vial Developer (Lyophilised) Purple 1 vial -
Research areas
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Relevance
Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. -
Alternative names
- Isocitric acid
Images
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Functional Studies - Isocitrate Assay Kit (ab83424)Isocitrate standard curve generated using this kit protocol.