Invertase Activity Assay Kit (Colorimetric) (ab197005)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Other biological fluids, Purified protein, Saliva
- Sensitivity: 20 µU
Overview
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Product name
Invertase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Saliva, Other biological fluids, Purified protein -
Assay type
Enzyme activity -
Sensitivity
20 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Invertase Activity Assay Kit (Colorimetric) (197005) provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.
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Notes
Invertase (EC 3.2.1.26), also known as sucrase or β -fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glucose Standard (100 mM) 1 x 100µl Invertase Assay Buffer 1 x 25ml Invertase Enzyme Mix 1 vial Invertase Hydrolysis Buffer 1 x 25ml Invertase Stop Solution 1 x 1ml Invertase Substrate 1 x 0.2ml OxiRed Probe Red 1 x 0.2ml Positive Control 1 vial
Images
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Invertase Activity Assay Kit (ab197005)
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Invertase Activity Assay Kit (ab197005)Detection of bacterial Invertase activity in human saliva. Samples collected: 1-Fasting; 2-Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 μl and 3 = 10 μl) were incubated for 20 min with Invertase substrate. Assay was performed following the kit protocol.
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