Human VEGF-D ELISA Kit (ab233625)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 75.7 pg/ml
- Range: 348.2 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGF-D ELISA Kit
See all VEGFD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% VEGF-D/serum 5 2.9% Inter-assay Sample n Mean SD CV% VEGF-D/serum 3 2.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
75.7 pg/ml -
Range
348.2 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88 85% - 92% Cell culture media 101 98% - 106% Hep Plasma 78 74% - 82% Cit plasma 88 84% - 92% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human VEGF-D ELISA Kit (ab233625) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF-D protein in hep plasma, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF-D with 75.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human VEGF-D Capture Antibody 1 x 600µl 10X Human VEGF-D Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human VEGF-D Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
Tissue specificity
Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Post-translational
modificationsUndergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- c-fos induced growth factor
- c-fos induced growth factor (vascular endothelial growth factor D)
- c-fos-induced growth factor
see all -
Database links
- Entrez Gene: 2277 Human
- Omim: 300091 Human
- SwissProt: O43915 Human
- Unigene: 11392 Human
Images
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Other - Human VEGF-D ELISA Kit (ab233625)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human VEGF-D standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native VEGF-D in human serum and plasma samplesThe concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, and plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,105 pg/mL in neat serum, 1,349 pg/mL in neat plasma (citrate) and 1,232 pg/mL in neat plasma (heparin). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).
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Interpolated concentrations of spike VEGF-D in human serum, plasma, and cell culture media samplesThe concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, and RPMI cell culture media containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,193 pg/mL with a range of 956.9 – 3,270 pg/mL.
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Sandwich ELISA - Human VEGF-D ELISA Kit (ab233625)To learn more about the advantages of recombinant antibodies see here.
