Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human USP14 (TGT) knockout HeLa cell pellet (ab279078)

Overview

  • Product name

    Human USP14 (TGT) knockout HeLa cell pellet
    See all USP14/TGT kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).

  • Sequence similarities

    Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
    Contains 1 ubiquitin-like domain.

  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • Alternative names

    • Deubiquitinating enzyme 14
    • TGT
    • tRNA guanine transglycosylase
    • tRNA guanine transglycosylase 60 kD subunit
    • Ubiquitin carboxyl terminal hydrolase 14
    • Ubiquitin carboxyl-terminal hydrolase 14
    • Ubiquitin specific peptidase 14
    • Ubiquitin specific processing protease 14
    • Ubiquitin specific protease 14
    • Ubiquitin thiolesterase 14
    • Ubiquitin-specific-processing protease 14
    • UBP14_HUMAN
    • USP 14
    • USP14
    see all

Properties

Images

  • Sanger Sequencing - Human USP14 (TGT) knockout HeLa cell pellet (ab279078)
    Sanger Sequencing - Human USP14 (TGT) knockout HeLa cell pellet (ab279078)
    Homozygous: 1 bp insertion in exon 1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

Sign up