Human ULBP2 ELISA Kit (ab267654)
Key features and details
- Sensitivity: 13 pg/ml
- Range: 12.29 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ULBP2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
12.29 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122.7 107% - 143% Plasma 122.3 101% - 145% Cell culture media 113.1 110% - 116% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ULBP2 ELISA Kit is designed for the quantitative determination of ULBP2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human ULBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ULBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ULBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ULBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAMTS-13, Aggrecan, Angiotensinogen, B7- H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM- 1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7 (CD328), Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human ULBP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human ULBP2 Detection Antibody 2 vials Human ULBP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. -
Tissue specificity
Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. -
Sequence similarities
Belongs to the MHC class I family. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Alternative names
- ALCAN alpha
- ALCAN-alpha
- N2DL 2
see all -
Database links
- Entrez Gene: 80328 Human
- Omim: 605698 Human
- SwissProt: Q9BZM5 Human
- Unigene: 656778 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
