Human TREM2 ELISA Kit (ab224881)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.5 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TREM2 ELISA Kit
See all TREM2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 6.3% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
10.5 pg/ml -
Range
78.1 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 85% - 104% Cell culture extracts 109 100% - 117% Tissue Extracts 99 95% - 101% Cell culture media 91 86% - 94% Hep Plasma 103 97% - 111% EDTA Plasma 101 93% - 105% Cit plasma 95 90% - 103% Cerebral Spinal Fluid 111 109% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human TREM2 ELISA Kit (ab224881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM2 protein in cell culture extracts, cell culture media, cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TREM2 with 10.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.
SPECIES REACTIVITY This kit recognizes Human TREM2 protein.
Other species reactivity was determined by measuring 25% serum samples of various species, interpolating the protein concentrations from the Human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in Human serum assayed at the same dilution.
Reactivity was determined for the following species: Mouse, Rat and cow. -
Notes
TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer’s disease. Ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human TREM2 Capture Antibody 1 x 600µl 10X Human TREM2 Detector Antibody 1 x 600µl Human TREM2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- TREM 2
- TREM-2
- TREM2
see all -
Database links
- Entrez Gene: 54209 Human
- Omim: 605086 Human
- SwissProt: Q9NZC2 Human
- Unigene: 435295 Human
Images
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Other - Human TREM2 ELISA Kit (ab224881)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human TREM2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Human TREM2 standard curve in Sample Diluent NS.Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
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Interpolated concentrations of native TREM2 in Human serum and plasma samples.The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).
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Serum from ten individual healthy Human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.
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Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.
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Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/µg in THP-1 cell extract and 0.34 pg/µg in placenta tissue extract.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)To learn more about the advantages of recombinant antibodies see here.
