Human TRAIL R2 ELISA Kit (ab233614)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.1 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TRAIL R2 ELISA Kit
See all DR5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HepG2 cells 9 1.9% Inter-assay Sample n Mean SD CV% HepG2 cells 3 3.5% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.1 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91 90% - 93% Cell culture extracts 100 99% - 100% Tissue Extracts 105 104% - 105% EDTA Plasma 81 80% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TRAIL R2 ELISA Kit (ab233614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAIL R2 protein in edta plasma, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TRAIL R2 with 10.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TRAIL R2 Capture Antibody 1 x 600µl 10X Human TRAIL R2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human TRAIL R2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
Tissue specificity
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
Involvement in disease
Squamous cell carcinoma of the head and neck -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Fas like protein
- Apoptosis inducing protein TRICK2A/2B
- Apoptosis inducing receptor TRAIL R2
see all -
Database links
- Entrez Gene: 8795 Human
- Omim: 603612 Human
- SwissProt: O14763 Human
- Unigene: 521456 Human
- Unigene: 661668 Human
Images
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Other - Human TRAIL R2 ELISA Kit (ab233614)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TRAIL R2 standard curve in 1X Cell Extraction Buffer PTRThe TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human TRAIL R2 standard curve in Sample Diluent NS.The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TRAIL R2 in human A549, MDA-MB-231, HepG2, and HT1080 cell extract samples.The concentrations of TRAIL R2 were measured in duplicates, interpolated from the TRAIL R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: A549 extract 250 µg/mL, MDA-MB-231 extract 62.5 µg/mL, HepG2 extract 62.5 µg/mL, and HT1080 extract 31.25 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TRAIL R2 in human pancreas and colon cancer extract samples.The concentrations of TRAIL R2 were measured in duplicate, interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are both 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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. Interpolated concentrations of spiked TRAIL R2 in human serum and plasma samples.The concentrations of TRAIL R2 were measured in duplicate and interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are follows: serum 50% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human TRAIL R2 ELISA Kit (ab233614)To learn more about the advantages of recombinant antibodies see here.
