Beta Lactamase Activity Assay Kit (Colorimetric) (ab197008)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Other biological fluids, Saliva, Serum, Urine
- Sensitivity: 0.06 mU/well
Overview
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Product name
Beta Lactamase Activity Assay Kit (Colorimetric)
See all Beta lactamase kits -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Other biological fluids -
Assay type
Enzyme activity -
Sensitivity
0.06 mU/well -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Beta Lactamase Activity Assay Kit (Colorimetric) ab197008 is a simple and sensitive assay that can detect and quantify the enzymatic activity of beta lactamases, a large family of hydrolases.
The beta lactamase assay protocol is based on the hydrolysis of the substrate Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (detectable at OD=490 nm), which is directly proportional to the amount of beta-lactamase activity.
This product can detect as low as 0.06 mU of beta-lactamase activity from bacterial samples or from biological fluids infected with beta-lactamase expressing bacteria.
Beta lacatmase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every 2-3 min for 30-60 min -
Notes
Beta Lactamases (β-Lactamases, βLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. βLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as β-lactams.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Beta Lactamase Assay Buffer 1 x 27ml Beta Lactamase Hydrolysis Buffer 1 x 100µl Nitrocefin 1 x 220µl Positive Control 1 vial -
Alternative names
- blaZ
- Penicillinase
Images
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Nitrocefin Standard Curve.
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Beta-Lactamase Activity.Beta-Lactamase activity in E. coli culture (5 µl), contaminated media (CM; 30 µl) and Positive Control (4 µl).
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Beta-Lactamase Activity.Beta-Lactamase Activity of E.coli and contaminated media expressed per milligram of protein.
