Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (ab257286)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260177 - Human TOP2B knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human TOP2B knockout HEK293T cell lysate (ab257286)Lane 1: Wild-type HEK-293T cell lysate (40µg)
Lane 2: TOP2B knockout HEK-293T cell lysate (40µg)
Lanes 1- 2: Merged signal (red and green). Green - ab125297 observed at 183 kDa. Red - loading control ab7291 observed at 50 kDa.
ab125297 Anti-Topoisomerase II beta/TOP2B antibody was shown to specifically react with Topoisomerase II beta/TOP2B in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266340 (knockout cell lysate ab257286) was used. Wild-type and Topoisomerase II beta/TOP2B knockout samples were subjected to SDS-PAGE. ab125297 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human TOP2B knockout HEK293T cell lysate (ab257286)Allele-1: 17 bp deletion in exon 3
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Sanger Sequencing - Human TOP2B knockout HEK293T cell lysate (ab257286)Allele-2: 13 bp deletion in exon 3