All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 knockout A549 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 77 kDa
Observed band size: 77 kDa

Lanes 1-2: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.

 ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

 

All lanes : Anti-Transglutaminase 2 antibody [EPR2956] (ab109121) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 knockout A549 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 77 kDa
Observed band size: 77 kDa

Lanes 1-2: Merged signal (red and green). Green - ab109121 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.

 ab109121 Anti-Transglutaminase 2 antibody [EPR2956] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109121 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

 

 

Homozygous: 1 bp insertion in exon6

Representative images of TGM2 knockout A549 cells, low and high confluency examples (top left and right respectively) and wild-type A549 cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.