Human Tenascin C ELISA Kit (ab277081)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.672 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Tenascin C ELISA Kit
See all Tenascin C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 12.3% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27.672 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 88% - 106% Hep Plasma 90 84% - 94% EDTA Plasma 98 96% - 99% Cit plasma 93 83% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Tenascin C ELISA kit (ab277081) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tenascin C protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tenascin C with 27.67 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tenascin C (TNC), is an extracellular matrix glycoprotein involved in tissue morphogenesis like embryogenesis, wound healing and tumorigenesis. Tenascin C is implicated in guidance of migrating neurons and axons during development, synaptic plasticity and neuronal regeneration. Tenascin C also as a role in neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. In tumors, Tenascin C stimulates angiogenesis by elongation, migration and sprouting of endothelial cell. Tenascin C shares 69.0%, 70.6%, 72.0%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Tenascin C Capture Antibody 1 x 600µl 10X Human Tenascin C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Tenascin C Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
Sequence similarities
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- 150 225
- Cytotactin
- Glioma associated extracellular matrix antigen
see all -
Database links
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Tenascin C standard curve in Sample Diluent NS. The Tenascin C standard curve was prepared as described in Section 10.The Tenascin C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Tenascin C in human serum and plasma samples.The concentrations of Tenascin C were measured in duplicates, interpolated from the Tenascin C standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 153.25 ng/mL in serum, 113.37 ng/mL in plasma (citrate), 108.74 ng/mL in plasma (EDTA), and 101.20 ng/mL in plasma (heparin).
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5% serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 351.07 ng/mL with a range of 247.61 – 487.97 ng/mL.
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Sandwich ELISA - Human Tenascin C ELISA KitTo learn more about the advantages of recombinant antibodies see here.
