Human SOX9 ELISA Kit (ab253226)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 22.8 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SOX9 ELISA Kit
See all SOX9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 6% Inter-assay Sample n Mean SD CV% Extract 3 2.3% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
22.8 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 105 102% - 112% Cell Lysate 96 94% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SOX9 ELISA Kit (ab253226) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOX9 protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SOX9 with 22.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
SOX9 is a transcriptional regulator that plays a role in the differentiation of chondrocytes and skeletal development. Human, mouse and rat have high SOX9 protein sequence homology.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SOX9 Capture Antibody 1 x 600µl 10X Human SOX9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human SOX9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. -
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- campomelic dysplasia autosomal sex reversal
- CMD 1
- CMD1
see all -
Database links
- Entrez Gene: 6662 Human
- Omim: 608160 Human
- SwissProt: P48436 Human
- Unigene: 647409 Human
Images
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Other - Human SOX9 ELISA Kit (ab253226)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SOX9 standard curve in 1X Cell Extraction Buffer PTR.The SOX9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SOX9 in human HepG2 cell extract load, testis tissue extract load and brain tissue extract load (100 µg/mL, 1,000 µg/mL, 1,000 µg/mL respectively)The concentrations of SOX9 were measured in duplicate and interpolated from the SOX9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOX9 concentration was determined to be 3,573 pg/mL in HepG2 cell extract, 5,161 pg/mL in testis tissue extract, and 458.7 pg/mL in brain tissue extract.
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Other species reactivity was determined by measuring brain tissue extract samples of various species, interpolating the protein concentrations from the human standard curve.Protein concentrations are expressed in pg of SOX9 protein per mg of extract. The mean SOX9 concentration was determined to be 458.7 pg/mg in human brain tissue extract, 461.0 pg/mg in mouse brain tissue extract, and 466.2 pg/mg in rat brain tissue extract.
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Sandwich ELISA - Human SOX9 ELISA Kit (ab253226)To learn more about the advantages of recombinant antibodies see here.
