Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human SOD2 ELISA Kit (ab178012)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.22 ng/ml
  • Range: 0.78 ng/ml - 50 ng/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human SOD2 ELISA Kit
    See all SOD2/MnSOD kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HepG2 5 3.8%
    Inter-assay
    Sample n Mean SD CV%
    HepG2 3 4.2%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.22 ng/ml

  • Range

    0.78 ng/ml - 50 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 92.1 85.5% - 95.6%
    Fetal Bovine Serum 79 75.7% - 82%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human SOD2 (Superoxide Dismutase 2) ELISA kit (ab178012) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOD2 protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human SOD2 with 220 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • Notes

    The principle cellular anti-oxidant enzymes are the superoxide dismutase family (SOD, E.C. 1.15.1.1). These enzymes dismutate superoxide into hydrogen peroxide which is further detoxified by other cellular defenses such as glutathione peroxidase and catalase. Superoxide and its products have been implicated in a wide range of diseases including cancer, inflammation, neurodegenerative diseases, diabetes and aging. The SOD family has 3 members, two of which are Cu-Zn type – the extracellular SOD3 and the cytoplasmic SOD1. The other member is the mitochondrial Mn (manganese) type SOD2. The mitochondrial Mn-SOD2 is a homotetramer of subunit mass 23 kDa in the mitochondrial matrix. SOD2 has been shown to be essential since knockout mice die shortly after birth. SOD2 levels may be downregulated in tumor cells and studies show that over expression of SOD2 in tumor cells may suppress cell division and cancer growth (Oberley, Biomedecine & Pharmacotherapy, 2005, 59, p143-8).

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

Images

  • Other - Human SOD2 ELISA Kit (ab178012)
    Other - Human SOD2 ELISA Kit (ab178012)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of SOD2 standard curve.
    Example of SOD2 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of HepG2 cell lysate within the working range of the assay.
    Titration of HepG2 cell lysate within the working range of the assay.

    Background-subtracted data values from triplicate measurements (mean +/- SD) are graphed.

  • Comparison of SOD2 expression in various cell lines (HepG2, HeLa, Jurkat, MDA-MB-453 (MDA453) and SH-SY5Y (SH-5Y)) using SOD2 Human SimpleStep™ ELISA Kit.
    Comparison of SOD2 expression in various cell lines (HepG2, HeLa, Jurkat, MDA-MB-453 (MDA453) and SH-SY5Y (SH-5Y)) using SOD2 Human SimpleStep™ ELISA Kit.

    Background-subtracted data values from triplicate measurements of two lysate concentrations (3 and 1.5 µg/mL) are graphed as mean +/- SD.

  • Quantification of SOD2 expression in various cell lines
    Quantification of SOD2 expression in various cell lines

    Quantification of SOD2 expression in various cell lines (HepG2, HeLa, Jurkat, MDA-MB-453 (MDA453) and SH-SY5Y (SH-5Y)) using SOD2 Human SimpleStep ELISA KitTM.

  • Comparison of SOD2 expression in various cell lines by Western blotting.
    Comparison of SOD2 expression in various cell lines by Western blotting.

    Cell lysates (20 µg) of HepG2 (lane 2), HeLa (lane 3), Jurkat (lane 4), MDA-MB-453 (lane 5) and SH-SY5Y (lane 6) were analyzed by Western blotting using ab13533 as primary antibody. Lane 1 shows migration of molecular weight marker.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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