Human SIPA1 (Spa-1) knockout HeLa cell lysate (ab258189)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262337 - Human SIPA1 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human SIPA1 knockout HeLa cell lysate (ab258189)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: SIPA1 knockout HeLa cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: HEK-293 cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab189929 observed at 130 kDa. Red - loading control ab7291 observed at 50 kDa.
ab189929 Anti-Spa-1 antibody [EPR14134] was shown to specifically react with Spa-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265434 (knockout cell lysate ab258189) was used. Wild-type and Spa-1 knockout samples were subjected to SDS-PAGE. ab189929 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SIPA1 knockout HeLa cell lysate (ab258189)Allele-1: 4 bp deletion in exon5
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Sanger Sequencing - Human SIPA1 knockout HeLa cell lysate (ab258189)Allele-2: 1 bp deletion in exon5
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Sanger Sequencing - Human SIPA1 knockout HeLa cell lysate (ab258189)Allele-3: 182 bp deletion in exon5
