Human SFN (14-3-3 sigma) knockout HeLa cell line (ab261779)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human SFN knockout HeLa cell line (ab261779)All lanes : Anti-14-3-3 sigma/SFN antibody [1433S01] (ab14123) at 1/200 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SFN knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab14123 observed at 28 kDa. Red - loading control, ab52866 observed at 50 kDa.
ab14123 Anti-14-3-3 sigma/SFN antibody [1433S01] was shown to specifically react with 14-3-3 sigma/SFN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261779 (knockout cell lysate ab257288) was used. Wild-type and 14-3-3 sigma/SFN knockout samples were subjected to SDS-PAGE. ab14123 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (ab52866) were incubated overnight at 4°C at 1 in 200 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SFN knockout HeLa cell line (ab261779)Allele-1: 2 bp deletion in exon 1.
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Sanger Sequencing - Human SFN knockout HeLa cell line (ab261779)Allele-2: 1 bp deletion in exon 1.