Human Prolactin/PRL ELISA Kit (ab226901)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.64 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Prolactin/PRL ELISA Kit
See all Prolactin/PRL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 14% Inter-assay Sample n Mean SD CV% Serum 3 12.6% -
Sample type
Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17.64 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 106 100% - 109% Serum 89 86% - 91% Cell culture media 114 108% - 118% Hep Plasma 90 88% - 91% EDTA Plasma 79 76% - 82% Cit plasma 76 70% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Prolactin/PRL ELISA Kit (ab226901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin/PRL protein in cit plasma, edta plasma, hep plasma, milk, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Prolactin/PRL with 17.64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Prolactin Capture Antibody 1 x 600µl 10X Human Prolactin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Human Prolactin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. -
Cellular localization
Secreted -
Alternative names
- Decidual prolactin
- GHA1
- Growth hormone A1
see all -
Database links
- Entrez Gene: 5617 Human
- Omim: 176760 Human
- SwissProt: P01236 Human
- Unigene: 1905 Human
Images
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Other - Human Prolactin/PRL ELISA Kit (ab226901)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Prolactin Competitor standard curve comparison -
Example of human Prolactin standard curve in Sample Diluent 25BSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Prolactin in human serum and plasma and of spiked Prolactin in cell culture media samplesThe concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 1,066 pg/mL in serum, 480 pg/mL in plasma (citrate), 801 pg/mL in plasma (heparin), and 711 pg/mL in plasma (EDTA).
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Interpolated concentrations of native Prolactin in human pregnant serum and milkThe concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 42,647 pg/mL in pregnant serum and 1,214 pg/mL in milk.
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Serum from ten individual healthy human female donors and ten individual healthy male donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration in females was determined to be 2,778 pg/mL with a range of 294 – 18,132 pg/mL. The mean Prolactin concentration in males was determined to be 1,733 pg/mL with a range of 179 – 7,626 pg/mL.
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Sandwich ELISA - Human Prolactin/PRL ELISA Kit (ab226901)To learn more about the advantages of recombinant antibodies see here.
