Human PRAME ELISA Kit (ab234561)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 83 pg/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PRAME ELISA Kit
See all PRAME kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 5 4.9% Inter-assay Sample n Mean SD CV% Cell extract 3 3% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
83 pg/ml -
Range
0.313 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 105 104% - 106% Tissue Extracts 95 94% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PRAME ELISA Kit (ab234561) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PRAME protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PRAME with 83 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PRAME Capture Antibody 1 x 600µl 10X Human PRAME Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Denaturant 1 x 500µl Human PRAME Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. -
Tissue specificity
Expressed in testis. Detected in samples of kidney, brain and skin. -
Sequence similarities
Belongs to the PRAME family.
Contains 4 LRR (leucine-rich) repeats. -
Cellular localization
Nucleus. Cell membrane. - Information by UniProt
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Alternative names
- 4930534P07Rik
- Cancer/testis antigen 130
- CT130
see all -
Database links
- Entrez Gene: 23532 Human
- Omim: 606021 Human
- SwissProt: P78395 Human
- Unigene: 30743 Human
Images
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Other - Human PRAME ELISA Kit (ab234561)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PRAME standard curve in 1X Cell Extraction Buffer + Enhancer.The PRAME standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PRAME in human K562 cell extract based on a 100 µg/mL extract load and testis tissue extract based on a 180 µg/mL extract load.The concentrations of PRAME were measured in duplicate and interpolated from the PRAME standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PRAME concentration was determined to be 14.70 ng/mL in K562 cell extract and 2.359 ng/mL in testis tissue extract.
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Sandwich ELISA - Human PRAME ELISA Kit (ab234561)To learn more about the advantages of recombinant antibodies see here.