Human PEDF Antibody Pair - BSA and Azide free (ab256680)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human PEDF Antibody Pair - BSA and Azide free
See all PEDF kits -
Assay type
ELISA set -
Range
468.75 pg/ml - 30000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human PEDF. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246535), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human PEDF Capture Antibody (unconjugated) 1 x 100µg Human PEDF Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Retinal pigment epithelial cells and blood plasma. -
Sequence similarities
Belongs to the serpin family. -
Developmental stage
Expressed in quiescent cells. -
Domain
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
Post-translational
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Alternative names
- Cell proliferation-inducing gene 35 protein
- EPC 1
- EPC-1
see all -
Database links
- Entrez Gene: 5176 Human
- Omim: 172860 Human
- SwissProt: P36955 Human
- Unigene: 532768 Human
Images
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Sandwich ELISA - Human PEDF Antibody Pair - BSA and Azide free (ab256680)To learn more about the advantages of recombinant antibodies see here.
