Human PDK4 knockout HeLa cell lysate (ab257217)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260899 - Human PDK4 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human PDK4 knockout HeLa cell lysate (ab257217)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: PDK4 knockout HeLa cell lysate (20 µg)
Lane 3: Human heart tissue lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab110336 observed at 48 kDa. Red - loading control, ab129002 observed at 124 kDa.
ab110336 Anti-PDK4 antibody [1C2BG5] was shown to specifically react with PDK4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261805 (knockout cell lysate ab257217) was used. Wild-type and PDK4 knockout samples were subjected to SDS-PAGE. ab110336 and Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 1000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human PDK4 knockout HeLa cell lysate (ab257217)Allele-1: 1 bp deletion in exon 1
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Sanger Sequencing - Human PDK4 knockout HeLa cell lysate (ab257217)Allele-2: 1 bp insertion in exon 1
