Human PDGF R alpha ELISA Kit (ab242246)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.89 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PDGF R alpha ELISA Kit
See all PDGFR alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.6% Inter-assay Sample n Mean SD CV% serum 3 7.7% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.89 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 105% - 107% Serum 92 84% - 97% Cell culture extracts 90 85% - 97% Tissue Extracts 98 91% - 105% Hep Plasma 99 94% - 105% EDTA Plasma 97 93% - 102% Cit plasma 102 100% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PDGF R alpha ELISA Kit (ab242246) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF R alpha protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF R alpha with 1.89 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PDGF R alpha Capture Antibody 1 x 600µl 10X Human PDGF R alpha Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human PDGF R alpha Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Alpha-type platelet-derived growth factor receptor
- CD140 antigen-like family member A
- CD140a
see all -
Database links
- Entrez Gene: 5156 Human
- Omim: 173490 Human
- SwissProt: P16234 Human
- Unigene: 74615 Human
Images
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Other - Human PDGF R alpha ELISA Kit (ab242246)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PDGF R alpha standard curve in Sample Diluent NS.The PDGF R alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human PDGF R alpha standard curve in 1X Cell Extraction Buffer PTR.The PDGF R alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native PDGF R alpha in human serum, and plasma samples.The concentrations of PDGF R alpha were measured in duplicates, interpolated from the PDGF R alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 5932.99 pg/mL in serum, 5414.30 pg/mL in plasma (citrate), 5256.55 pg/mL in plasma (EDTA), and 4851.59 pg/mL plasma (heparin). -
Interpolated concentrations of native PDGF R alpha in human SH-SY5Y based on a 125 ug/mL load.Interpolated concentrations of native PDGF R alpha in human placenta, and skeletal muscle extract samples based on a 250 μg/mL extract load. The concentrations of PDGF R alpha were measured in duplicate and interpolated from the PDGF R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 1105.74 pg/mL in SH-SY5Y extract, 1507.92 pg/mL in placenta extract and 1521.98 pg/mL in skeletal muscle extract.
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Serum (10%) from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF R alpha concentration was determined to be 7160.36 pg/mL with a range of 4446.95 - 12638.24 pg/mL.
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Sandwich ELISA - Human PDGF R alpha ELISA Kit (ab242246)To learn more about the advantages of recombinant antibodies see here.
