Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.65 pg/ml
- Range: 0.69 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human PDGF BB ELISA Kit, Fluorescent
See all PDGF B kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 5.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.65 pg/ml -
Range
0.69 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 101% - 106% Serum 95 85% - 100% Hep Plasma 104 92% - 114% EDTA Plasma 99 91% - 107% Cit plasma 109 100% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
PDGF BB in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PDGF BB protein in human serum, plasma, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
-
Notes
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human PDGF BB Capture Antibody 1 x 600µl 10X Human PDGF BB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 4BI 1 x 6ml Human PDGF BB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
- Information by UniProt
-
Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
Images
-
Other - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
. Example of human PDGF BB standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Titration of human serum within the working range of the assay.Background subtracted data from duplicate measurements are plotted.
-
Observed PDGF-BB levels in pooled donor normal human plasma and normal human platelet-poor plasma (PP-Plasma).Mean human PDGF-BB values fall within expected normal reference ranges (WHO).
-
Observed PDGF-BB levels in individual donor normal Human serum (n=10).Mean human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
-
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours aliquoted and assayed for endogenous PDGF-BB levels.
-
The PDGF-BB SimpleStep ELISA was validated for assay specificity against PDGF-A protein.Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
