Human PDGF BB ELISA Kit (ab184860)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.1 pg/ml
- Range: 2.06 pg/ml - 1500 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PDGF BB ELISA Kit
See all PDGF B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human Serum 8 3.3% Inter-assay Sample n Mean SD CV% Human Serum 3 5.3% -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
2.1 pg/ml -
Range
2.06 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 85% - 100% Cell culture media 104 101% - 106% Hep Plasma 104 92% - 114% EDTA Plasma 99 91% - 107% Cit plasma 109 100% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PDGF BB ELISA kit (ab184860) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF BB protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human PDGF BB with 2.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PDGF BB Capture Antibody 1 x 600µl 10X Human PDGF BB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PDGF BB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
Images
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Other - Human PDGF BB ELISA Kit (ab184860)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Typical Standard CurveExample of PDGF-BB standard curve for ab184860
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Titration of Human SerumTitration of Human serum with ab184860 within the working range of the assay. Background subtracted data from duplicate measurements are plotted.
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PDGF-BB levels in Human plasmaObserved PDGF-BB levels in pooled donor normal Human plasma and normal Human platelet-poor plasma (PP-Plasma). Mean Human PDGF-BB values fall within expected normal reference ranges (WHO).
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Assay Specificity of ab184860ab184860 was validated for assay specificity against PDGF-A protein. Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
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PDGF-BB Levels in Human SerumObserved PDGF-BB levels in individual donor normal Human serum (n=10). Mean Human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
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Assay Specificity of ab184860Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous PDGF-BB levels.
