Human PDGF AB ELISA Kit (ab100623)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human PDGF AB (lyophilized) 2 vials PDGF AB Microplate (12 x 8 wells) 1 unit Recombinant Human PDGF AB Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain. -
Alternative names
- PDGF A chain
- PDGF B chain
- PDGF1
see all -
Database links
- Entrez Gene: 5154 Human
- Entrez Gene: 5155 Human
- SwissProt: P04085 Human
- SwissProt: P01127 Human
- Unigene: 535898 Human
- Unigene: 1976 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab100623.
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Typical Standard CurveRepresentative Standard Curve using ab100623.