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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Als2 antibody [EPR11185] (ab170896)

Price and availability

314 937 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Als2 antibody [EPR11185] (ab170896)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11185] to Als2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Als2 antibody [EPR11185]
    See all Als2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11185] to Als2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Als2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96Q42

  • Positive control

    • SH-SY5Y, 293T and HeLa cell lysates; Human brain and pancreas tissues; SH-SY5Y cells; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11185
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Als2 antibody [EPR11185] (ab170896)
    Immunocytochemistry/ Immunofluorescence - Anti-Als2 antibody [EPR11185] (ab170896)

    Immunofluorescent analysis of SH-SY5Y cells labeling Als2 with ab170896 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-Als2 antibody [EPR11185] (ab170896)
    Flow Cytometry - Anti-Als2 antibody [EPR11185] (ab170896)

    Flow cytometric analysis of permeabilized 293T cells labeling Als2 with ab170896 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Als2 with ab170896 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Als2 antibody [EPR11185] (ab170896)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Als2 with ab170896 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Als2 antibody [EPR11185] (ab170896)
    Western blot - Anti-Als2 antibody [EPR11185] (ab170896)
    All lanes : Anti-Als2 antibody [EPR11185] (ab170896) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 184 kDa

  • Anti-Als2 antibody [EPR11185] (ab170896)
    Anti-Als2 antibody [EPR11185] (ab170896)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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